Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------CLGVLLRNDGRKPILGFEPFRIRR-----TVSFPGYDFVLKVGRRCITEK---------ISGTIPPHLHVHVVWGFPAPVR--PVCNVYNGWTERFERYGRNVREAPW------------- 1V95 Chain:A ((1-130)) GSSGSSGPVDCSVIVVNKQTKDYAESVGRKVRDLGMVVDLIFLNTEVSLSQALEDVSRGGSPFAIVITQQHQIHRSCTVNIMFGTPQEHRNMPQADAMVLVARNYERYKNECREKEREEIARQASGPSSG

General information: TITO was launched using: RESULT: Template: 1V95.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 375 31314 83.50 340.37 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 83.50 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.106

(partial model without unconserved sides chains): PDB file : Tito_1V95.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1V95-query.scw PDB file : Tito_Scwrl_1V95.pdb: