Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WFHEGLSRHQAENLLMG-KEVGFFIIRASQSSPGDFSISVRHEDDVQHFKVMRDNKGNYFLWTEKFPSLNKLVDYY 2H46 Chain:E ((8-83)) WFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYH

General information: TITO was launched using: RESULT: Template: 2H46.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 215 -14764 -68.67 -196.85 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain E : 0.86 3D Compatibility (PKB) : -68.67 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.600

(partial model without unconserved sides chains): PDB file : Tito_2H46.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2H46-query.scw PDB file : Tito_Scwrl_2H46.pdb: