Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIHFSINKNLFLQALNITKRAISSKNAIPILSTVKIDVTNEGVTLIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDV-TLDFKEIEQNQIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRPILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKN-SDDFDVVIPSRSLREFSAVFTDDIETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMERARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLIDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN 4TR8 Chain:B ((16-383)) HMHFTIQREALLKPLQLVAGVVE---TLPVLSNVLLVVEGQQLSLTGTDLEVELVGRVVLE------DAAEPGEITVPARKLMDICKSLPNDVLIDIRVEEQ-KLLVKAGRSRFTLSTLPANDFP----GPGSLNFSIAQSKLRRLIDRTSFAMAQQDVRYYLNGMLLEVN-GGTLRSVATDGHRLAMCSLDAQIPSQDRHQVIVPRKGILELARLLTEQDGEVGIVLGQHHIRATTGEFTFTSKLVDGKFPDYERVLPRGGDKLVVGDRQQLREAFSRTAILSNEKYRG-IRLQLSNGLLKIQANNPEQEEAEEEVQV-EYNGGNLEIGFNVSYLLDVLGVIGTEQVRFILSDSNSSALVHEA-DNDDSAYVVMPMRL-

General information: TITO was launched using: RESULT: Template: 4TR8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1964 -37744 -19.22 -105.14 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -19.22 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.515

(partial model without unconserved sides chains): PDB file : Tito_4TR8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TR8-query.scw PDB file : Tito_Scwrl_4TR8.pdb: