Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKL-IANIKESTLYPILKKLEGNSFLTTYSRE-FQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGSIRHDKN 5H20 Chain:A ((9-106)) ---QMRKGMLEYCIMLLLHKEPAYASDIIQKLKEARLIVVEGTLYPLLTRLKNDDLLSYEWVESTQGPPRKYYKLTGKGESFLGELEASWKELNETVNHIA---------

General information: TITO was launched using: RESULT: Template: 5H20.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 267 -12333 -46.19 -128.47 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -46.19 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.409

(partial model without unconserved sides chains): PDB file : Tito_5H20.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5H20-query.scw PDB file : Tito_Scwrl_5H20.pdb: