Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVTMQYSCGKININIPDGYGDIKDIVFSAHIIVRYN-NGHCGGIDPHIIGLCKKQIRRMSLYPILIIVSRDSKVI----DDYKNLDIAYVDCTQCSN--------NFET-ALHVKN--ILKLLKIQLIHCHGYSTNYFLYMLKKLDKNGFGKVKTVITCHGWVEYNLK---KKFLTYFDFWTYSMGDAFICVSETMKKRLESIIK--NKKIVAINNGINVSNSDLDVV-GVQDFKKEFCIPNNKKIICYVGRLDPEKRPDRFLEFAEKLFLVRE--DVIFIMAGNGSMWAALKEKICHLKCRDNFRLLGEIYPAT--IVYQISDLLYIPSDTEGIPMCVLESMSQGTSVLASNVGGLSEIIEHRVDGFLFEKEDVEGVCACANFLLNDSEYLKYIGENSKSKIRKHFSVQKMFVETMRVYDELLEKSSHG 3C48 Chain:B ((21-430)) ----------------------MRVAMISMHTSPLQQ-----GMNVYILSTATELAKQG--IEVDIYTRATRPSQGEIVRVAENLRVINIAAGPYEGLSKEELPTQLAAFTGGMLSFTRREKVTYDLIHSHYWLSGQVGWLLRD-----LWRIPLIHTAHTLAAVK--TPESEARRICEQQLVDNADVLAVNTQEEMQDLMHHYDADPDRISVVSPGADVELYSPGNDRATERSRRELGIPLHTKVVAFVGRLQPFKGPQVLIKAVAALFDRDPDRNLRVIICGGPS---TYRHMAEELGVEKRIRFLDPRPPSELVAVYRAADIVAVPSFNESFGLVAMEAQASGTPVIAARVGGLPIAVAEGETGLLVDGHSPHAWADALATLLDDDETRIRMGEDAVEHA-RTFSWAATAAQLSSLYNDAIANEN--

General information: TITO was launched using: RESULT: Template: 3C48.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1942 -16889 -8.70 -46.65 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : -8.70 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.532

(partial model without unconserved sides chains): PDB file : Tito_3C48.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3C48-query.scw PDB file : Tito_Scwrl_3C48.pdb: