Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKEVANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVWTEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESAS-TDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIHSSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEEVSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVRLVPGLEPHDVLEKIRKQLDKNGFDKVELYYTL--GEMSYRSDMSAPAILNVIELAKKFYPQGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVEELIRSYE 5UEJ Chain:B ((5-378)) -----------------TETQSLELAKELISRPSVTPD---DRDCQKLLAERLHKIGFAAEELHFGDTKNIWLRRGT---KAPVVCFAGHTDVVPTGPVEKWDSPPFEPAERDGRLYGRGAADMKTSIACFVTACERFVAKHPNHQGSIALLITSDEEGDALDGTTKVVDVLKARDELIDYCIVGEPTAVDKLGDMIKNGRRGSLSGNLTVKG--KQGHIAYPHLAINPVHTFAPALLELTQEVWDE------------------------GN-----------E--------------YFPPTSFQISNINGGT---GATNVIPGELNVKFNFRFSTESTEAGLKQRVHAILDKHG---VQYDLQWSCSGQPFLTQ-AGKLTDVARAAIAETCGI-EAELSTTGGTSDG-RFIKAIAQELIELGPSN--ATIHQINENVRLNDIPKLSAVYEGILARL-

General information: TITO was launched using: RESULT: Template: 5UEJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2173 -9355 -4.30 -25.21 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : -4.30 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.425

(partial model without unconserved sides chains): PDB file : Tito_5UEJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UEJ-query.scw PDB file : Tito_Scwrl_5UEJ.pdb: