Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTL-IVAISIIFMQTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIRIAE 2CBL Chain:A ((218-305)) THPGYMAFLTYDEVKARLQKFIHKPGSYIFRLSCTRLGQWAIGYVTADGNILQTIPHNKPLFQALIDGFRE---------------GFYLFPDGRNQNPDLTG-----

General information: TITO was launched using: RESULT: Template: 2CBL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 333 5261 15.80 61.89 target 2D structure prediction score : 0.31 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 15.80 2D Compatibility (Sec. Struct. Predict.) : 0.31 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.287

(partial model without unconserved sides chains): PDB file : Tito_2CBL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CBL-query.scw PDB file : Tito_Scwrl_2CBL.pdb: