Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRQTPSLSFEVFPPTPAVGNDNIISALQDMQELAPHFISVTASNNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALRGDIIPDVEPQKDFRYATDLIELIKERAPHFDIIGACYPEGHPDSPNQISDIQNLKKKVDAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIHLPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDIAGVHLYTMNNADTAKYIHQATHALFNHQSLG 1B5T Chain:B ((4-266)) ------NVSFEFFPPRTSEMEQTLWNSIDRLSSLKPKFVSVTYGANSGE-RDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTIARDYWNNGIRHIVALRGDLPPG--SGKPEMYASDLVTLLKEVA-DFDISVAAYPEVHPEAKSAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFKQAKKFADMT-NVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYTLNRAEMSYAI--------------

General information: TITO was launched using: RESULT: Template: 1B5T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1289 -172793 -134.05 -657.01 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -134.05 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_1B5T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1B5T-query.scw PDB file : Tito_Scwrl_1B5T.pdb: