Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLNKYIDHTLLKQDAKKKQIDSLLSEAREY--GFASVCVNPTWVEHAKKGL--EGT-DVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGN----LALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIV-VCQLAQKAGADFVKTSTGFSTGGATIADVTLMRETVGSDMGV------KAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADGDY 5EKY Chain:A ((13-216)) ----KLMDLTTLNDDDTDEKVIALCHQAKTPVGNTAAICIYPRFIPIARETLKEQGTPEIRIATVTNFPHGNDDIDIALAETRAAIAWGADEVDVVFPYRALMAGNEQVGFDLVKA--CKEACAAANVLLKVIIETGELKDEALIRKASEISIKAGADFIKTSTGKVAVNATPESARIMMEVI-RDMGVEKTVGFKPAGGVRTAEDAQKYL-------------------------

General information: TITO was launched using: RESULT: Template: 5EKY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 988 962 0.97 5.11 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 0.97 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.512

(partial model without unconserved sides chains): PDB file : Tito_5EKY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EKY-query.scw PDB file : Tito_Scwrl_5EKY.pdb: