Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------MNIVLLFLFSGGKMAKELQSSCYIVISFLVREMGIDIVEAISLMAELEKSGLVRLESSGD--LILKELGGAL 2J5O Chain:A ((740-811)) GSEGSEDDPLYDEAVRFVTESRRAS----------ISAVQRKLKIGYNRAARMIEAMEMAGVVTPMNTNGSREVIAPAPVRD

General information: TITO was launched using: RESULT: Template: 2J5O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 243 -6090 -25.06 -101.50 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -25.06 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.722

(partial model without unconserved sides chains): PDB file : Tito_2J5O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2J5O-query.scw PDB file : Tito_Scwrl_2J5O.pdb: