Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNHVASLYAEKVKLSLEDAGF--QVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVALGGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQPDGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVE-SILEHAETLIEHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGY-GKVMHGEAVAMGMVQISKIAEEKGLMPAGITQSITEMCQKFGLPVDYEN-WEVDKLYQALTHDKKARGNTLKLVLVPE-LGSATIHPV-SLEEMKDYLVK 3ZOK Chain:D ((17-373)) --VDVDLGDRSYPIYIGSGLLDQPDLLQRH-VHGKRVLVVTNSTVAPIYLDKVVGALTNENPNVSVESVILPDGEKYKNMDTLMKVFDKAIESRLDRRCTFVALGGGVIGDMCGYAAASFLRGVNFIQIPTTVMAQVDSSVGGKTGINHRLGKNLIGAFYQPQCVLIDTDTLNTLPDRELASGLAEVVKYGLIRDANFFEWQEKNMPALMARDPSALAYAIKRSCENKAEVVSLDEKESGLRATLNLGHTFGHAIETGFGYGQ-WLHGEAVAAGMVMAVDMSYRLGWIDESIVNRAHNILQQAKLPTAPPETMTVEMFKSVMAVDKKVADGLLRLILLKGPLGNCVFTGDYDRKALDETLH-

General information: TITO was launched using: RESULT: Template: 3ZOK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 2040 -55327 -27.12 -158.08 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain D : 0.80 3D Compatibility (PKB) : -27.12 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.534

(partial model without unconserved sides chains): PDB file : Tito_3ZOK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZOK-query.scw PDB file : Tito_Scwrl_3ZOK.pdb: