Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQETKLSA-KGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEIGAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDAKYAEYLAELDKEKPVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLL-ATGFTDTFRHVHGDVPERYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL 5DFJ Chain:A ((20-276)) LKICSWNVDGL---------RAWIKKKGLDWVKEEAPDILCLQQTKCSENKLPAE-----LQEL-PGLSHQYWSAPS-DKEGYSGVGLL-SRQCPLKVSY-GIGD-EEHDQEGRVIVAEFDSFVLVTAYVPNAGRGLVRLEYRQRWDEAFRKFLKGLASRKPLVLCGNLNVAHEEIDLRNPKGNKKNAGFTPQERQGFGELLQAVPLADSFRHLYPNTPYAYTFWTYM-MNARSKNVGWRLDYFLLSHSLLPALCDSKIRSKALGSDHCPITLYLAL

General information: TITO was launched using: RESULT: Template: 5DFJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1440 -26660 -18.51 -104.55 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -18.51 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.491

(partial model without unconserved sides chains): PDB file : Tito_5DFJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DFJ-query.scw PDB file : Tito_Scwrl_5DFJ.pdb: