Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANRKIVVALGGNAILSSDPSAK--AQQEALVETAKHLVKLIKNGDDLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDEGIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWRKVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFASQRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPKVEAAIAFVNGRPEGK-AVITSLENLGALIESESGTIIEKG 3KZF Chain:B ((4-316)) -AGKTVVIALGGNAMLQAKEKGDYDTQRKNVEIAASEIYKIHKAGYKVVLTSGNGPQVGAIKLQNQAA-AGVSPEMPLHVCGAMSQGFIGYMMSQAMDNVFCANNEPANCVTCVTQTLVDPKDQAFTNPTKPVGRFYTE------------ILREDAGRGWRVVVPSPRPLEIVEYGVIKTLIDNNVLVICTNGGGIPCKRENK-VISGVDAVIDKDLATSLLAKTLNSDYLMILTDVLNACI--KKPDERKLEEIKLSEILALEKDGHF--GSMGPKVRAAIEFT--QATGKMSIITSLSTAVDALNGKCGTRIIK-

General information: TITO was launched using: RESULT: Template: 3KZF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1621 15533 9.58 53.01 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.82 3D Compatibility (PKB) : 9.58 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.587

(partial model without unconserved sides chains): PDB file : Tito_3KZF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3KZF-query.scw PDB file : Tito_Scwrl_3KZF.pdb: