Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFGKILNKFKAWYKGDPGDMRWDPRTDTYVGTREPSKHWTAKVLSYFVD--FSLLIAKSIKKHPSA----------------YITQLLA--FIAILVSCFSIYLQYYVDDDEYKRCTIAH-----TNNQEIALKCKK-- 2YVQ Chain:A ((5-1478)) -----SSGHTAFLKAMLSTGFKIPQKGILIGIQQSFRPRFLGVAEQLHNEGFKLFATEATSDWLNANNVPATPVAWPSQELSSIRKLIRDGSIDLVINLPNNNTKFVHDNYVIRRTAVDSGIPLLTNFQVTKLFAEAVQ

General information: TITO was launched using: RESULT: Template: 2YVQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 399 -25774 -64.60 -240.87 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -64.60 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.302

(partial model without unconserved sides chains): PDB file : Tito_2YVQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YVQ-query.scw PDB file : Tito_Scwrl_2YVQ.pdb: