Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQYSMRFWKNKYFLLIMLCLGVISPSVYAQSFDQNFQEWKAKQQMYDQKLNISKSSHSNSSKISQTKNFNDSTGQIHLNQANVN-----------EFQQLKGVGEKKAQAIVEYRQKNGSFKNIDEIKNVK---GIGPAIFEKNKSRLAL 2HNH Chain:A ((811-880)) ------------------------------------------------------------------------ILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKGGYFRELFDLCARTDTKKLNRRVLE--------

General information: TITO was launched using: RESULT: Template: 2HNH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 136 11102 81.63 198.24 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 81.63 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.729

(partial model without unconserved sides chains): PDB file : Tito_2HNH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HNH-query.scw PDB file : Tito_Scwrl_2HNH.pdb: