Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRHIISVLVENEAGALSRLVGLFSQRNYNIETLNVAPTEDPTLSRLTLTTYGDDHKIEQITKQLNKLVEVEK--VVD--LS-EGSHIERELMLIKVKALG--ASRAEIKRTADIFRAQIVDVTPTTYTIQIAGTTEKLDGFIDALAENTILEVVRSGVSGIARGEKVLAI 1U8S Chain:B ((7-131)) --LVITAVGTDRPGICNEVVRLVTQAGCNIIDSRIAMFG--KEFTLLMLISGSPSNITRVETTLPLLGQQHDLITMMKRTSPHDHQTHAYTVEVYVESDDKLGLTEKFTQFFAQRQIGMASL------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1U8S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 340 -50481 -148.47 -454.78 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain B : 0.60 3D Compatibility (PKB) : -148.47 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.460

(partial model without unconserved sides chains): PDB file : Tito_1U8S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1U8S-query.scw PDB file : Tito_Scwrl_1U8S.pdb: