Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITHSLKDKVVLITGGAKNLGGLISRKFAEQGAKLAIHYNSSETQADAEQTLADVKALGADAILIQADLTDIDNIEKLFLDTKQHFGGIDIAINTVGKVLKKPILETTEQEFDSMSDINSKIAYFFIQSAGRHLNNGGKICSIVTSLLAAYT-GFYSTYEGLKAPVEHYTRTASKEFGDRQISVTAVAPGPMDTPFF-------------YGQESAEAVAYHKSASALGGLTKIEDIEPLVRFLVT-DGWWITGQTIFANGGYTTR 3QWF Chain:G ((16-268)) -----LDGKVALVTGSGRGIGAAVAVHLGRLGAKVVVNYANSTK--DAEKVVSEIKALGSDAIAIKADIRQVPEIVKLFDQAVAHFGHLDIAVSNSGVVSFGHLKDVTEEEFDRVFSLNTRGQFFVAREAYRHLTEGGRIVLTSSNTSKDFSVPKHSLYSGSKGAVDSFVRIFSKDCGDKKITVNAVAPGGTVTDMFHEVSHHYIPNGTSYTAEQRQQMAAHASPLHRNGWP--QDVANVVGFLVSKEGEWVNGKVLTLDGG----

General information: TITO was launched using: RESULT: Template: 3QWF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 1309 -64294 -49.12 -270.14 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain G : 0.78 3D Compatibility (PKB) : -49.12 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.480

(partial model without unconserved sides chains): PDB file : Tito_3QWF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3QWF-query.scw PDB file : Tito_Scwrl_3QWF.pdb: