Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKAQKLTLAVLISAAIISSAQASEQSEAKGFVEDANGSILFRTGYLTRDKKQGAKDTSSVAQSAIVSIESGFTPGIVGFGVGVVGDGSFK--IGENKNTGNQMIPKHNDGSAYDHWARGGGSVKARFSNTTVRYGTQVLDLPVLASNTGRMVPEYFTGTLLTSHEIKNLEVVAGKFTKDQMSDQINTDADA--------SGRGLDRAIVWGAKYKFNDNLNASYYGLDSKNALERHYANVNFKQPLANDSSLTYDFSGYHTKFDANAHTYSATGTVAPNYAADGIADEEKTNNIWAISGTYATGPHSVMLAYQQNTGNVGYDYGQNADGFQSIYLPNSY-MSDFIGNHEKSAQIQYNVDFGKLGVLPGLNWTTAFVYGWDIKVRNVTDDAQEREFFNQVKYTVQSGFAKDASLRIRNSYYRASDAYQGAYIGDTNEWRIFLDIPVKLF 2Y0L Chain:A ((12-402)) -----------------------------AGFLEDSKASLETRNFYMNRDFR----KREEWAQGFILNLQSGYTQG--TVGFGLDAMGMLGVKLDS-----------------PDTYSKLGLTAKVKVSQSELKVGTLIPKLPSVQPNNGRIFPQIFEGALLTSKEIKDLGFTAGRLEKTKI----DSEDLALNDKNGRFAGVSADHFDLGGLDYKLTDQLTASYHYSNLQDVYRQHFVGLLHSWPIGP-GELTSDLRFARSTDSGSAK------------------AGGIDNKSLNGMFTYSLGNHAFGAAWQRMNGDDAFPYLEG--SN-P-YLVNFVQVNDFAGPKERSWQLRYDYDFVGLGI-PGLTFMTRYVKGDNVELAGQ-GEGREWERNTELQYVFQSGALKNLGIRWRNATFRSNF------TRDIDENRLIVSYTLPIW

General information: TITO was launched using: RESULT: Template: 2Y0L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1958 66973 34.20 190.81 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 34.20 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.314

(partial model without unconserved sides chains): PDB file : Tito_2Y0L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Y0L-query.scw PDB file : Tito_Scwrl_2Y0L.pdb: