Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQLISLPKQILQCGQLQLDLSQPH-VMGILNVTPDSFSDGGKHNQLDQAVDHALSMIEQGATIIDIGGESTRPGASEVAVEEEVRRVVPVVEALSHH-NVILSIDTSQPEVIRAAKAAGAHIWNDV-RALTGPNALKTAVELDIPVVIMHMRGEPTTMNQLDQYTDVTLDVMQELQQRIDEALAAGVKKHNIIVDPGFGFAKNAQQNLKLLKEFWKLNELGYPILSGLSRKRFIGEALQGAPADQRAVGSVTGHLLSIQQGASIVRAHDVKEMHEAILVWKAMQQA 3TYE Chain:B ((27-291)) ----------LRCGEYTLNLNEKTLIMGILNV-------GGSYNEVDAAVRHAKEMRDEGAHIIDIGGEST-------SVEEEIKRVVPMIQAVSKEVKLPISIDTYKAEVAKQAIEAGAHIINDIWGAKAEPKIAEVAAHYDVPIILMHNR---DNMN----YRNLMADMIADLYDSIKIAKDAGVRDENIILDPGIGFAKTPEQNLEAMRNLEQLNVLGYPVLLGTSRKSFIGHVLD-LPVEERLEGTGATVCLGIEKGCEFVRVHDVKEMSRMAKMMDAM---

General information: TITO was launched using: RESULT: Template: 3TYE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1425 -4775 -3.35 -19.25 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -3.35 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.617

(partial model without unconserved sides chains): PDB file : Tito_3TYE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TYE-query.scw PDB file : Tito_Scwrl_3TYE.pdb: