Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MINIQNTILGKIVDRKHEDLAARLKQRNLQDVEELAKAATPVRGFANALQHKRPGVIAEIKKASPSKGIIRADFNPAEIAQQYEQAGAACLSVLTDV-DFFQGADENIAIARNHCTLPALRKDFLVDPYNVVEA-RALHADCILLIVACLSDQQLEEMSKTAFEHQLDVLVEVH-DEEELERALKLSEQCLLGVNNRNLKTF--DVDLNTTIRLKKLLPASRLLITESGIATPDDVRMMQEHDIHSFLVGESFMKQPRP--DQAFTALFGQPQTV 1PII Chain:? ((1-255)) ----MQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPST--RHFYDALQGARTAFILECKKASPSKGVIRDDFDPARIAAIYKH-YASAISVLTDEK-YFQGSFNFLPIVSQIAPQPILCKDFIIDPYQIYLAR-YYQADACLLMLSVLDDDQYRQLAAVAHSLEMGVLTEVSN-EEEQERAIALGAK-VVGINNRDLRDLSI--DLNRTRELAPKLGHNVTVISESGINTYAQVRELSH-FANGFLIGSALMAHD--DLHAAVRRVLLG----

General information: TITO was launched using: RESULT: Template: 1PII.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1376 -53927 -39.19 -217.45 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -39.19 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.456

(partial model without unconserved sides chains): PDB file : Tito_1PII.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1PII-query.scw PDB file : Tito_Scwrl_1PII.pdb: