Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTEKGVETMKIAVDAMGG-DHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKKYLTNEK---NITIIHTDEKINSD----D--EP-V--KAIRR---------KKTASMVLAAQAVKNGEADAVFSAG-NTGALLAAGLFIVGRIKNIERP-----GLMSTLPVVGKEGAGFDMLD-LGAN-----A-ENKPEHLLQYGILGSFYARKVRGIGRPRVALLNNGTE-------AT-KGSEVTKKAYELLQNETSL-NFIGNVEARELLN----GVADVVVT-DGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEMDYSKHGGAVLFGLKAPVIKTHGATGP------------DAVRYTIRQIHTMLETDVVGQLVEQFEKSEE 4JQP Chain:B ((15-341)) -------LPVIGITMGDAAGVGAEVVVKSLAHASVYA-QCRPLVIGDAKRLERANQIVGGEMKIRRIEDASEARYEQGTIDCIDLGLIPDDLPFGQLSAIAGDAAYQYIKRAVELAQSGKIDAICTAPLNKEALHAGGHKYPGHTEMLAHLTGVDEVSMMLVAPQ-L--RVIHVTTHIGIIDAIRKIEPGLVQRTIERGN---ATLVK-AGIERPRIGVCGINPHAGENGLFGYGEEEEKIIPAVTLLQER--GLDVTGPLPADTLFFRAGRGDFDLVVAMYHDQGHGPVKVL------------G-------------------------------LEAGVNVTVGLEVIRTSVDHGTAFDIAGKGVVDEGSMLEALRQGAELATR---------------

General information: TITO was launched using: RESULT: Template: 4JQP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1347 42146 31.29 158.44 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : 31.29 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.433

(partial model without unconserved sides chains): PDB file : Tito_4JQP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JQP-query.scw PDB file : Tito_Scwrl_4JQP.pdb: