Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSFGCWKEQKKNSKMSLEGEKMKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKYAIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINLPVFKGLDNTSLLYGAGTLSPDQEMG-KGNYALASHRATNPELLFTPLENLEMGAKIYLTDLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTSIKDATDDMRSAFNLETKTF 3RCC Chain:N ((10-154)) ---------------------------------------------------------------------------------------------------------HNLPVIGGIAIPDVEINLPIFKGLGNTELSYGAGTMKENQIMGGPNNYALASHHVF----LFSPLEHAKKGMKVYLTDKSKVYTYTITEISKVTPEHVEVIDDTPGKSQLTLVTCTDPEATERIIVHAELEKTGEFSTADE-------------

General information: TITO was launched using: RESULT: Template: 3RCC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain N - contact count / total energy / energy per contact / energy per residue : 645 -32961 -51.10 -242.36 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain N : 0.69 3D Compatibility (PKB) : -51.10 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.455

(partial model without unconserved sides chains): PDB file : Tito_3RCC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RCC-query.scw PDB file : Tito_Scwrl_3RCC.pdb: