Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERMKKSLVKLV----GEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNVSDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAKASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVTIEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS 2QIO Chain:D ((1-254)) MELLQGKTFVVMGVANQRSIAWGIARSLHNAGAKLIFTYAGERLERNVRELADTLEGQESLVLPCDVTNDEELTACFETIKQEVGTIHGVAHCIAFANRDDLKGEFVDTSRDGFLLAQNISAFSLTAVAREAKKVMTEGGNILTLTYLGGERVVKNYNVMGVAKASLEASVKYLANDLGQHGIRVNAISAGPIRTLSAKGVGDFNSILREIEERAPLRRTTTQEEVGDTAVFLFSDLARGVTGENIHVDSGYHI-

General information: TITO was launched using: RESULT: Template: 2QIO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1367 -83254 -60.90 -333.02 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain D : 0.87 3D Compatibility (PKB) : -60.90 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.621

(partial model without unconserved sides chains): PDB file : Tito_2QIO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QIO-query.scw PDB file : Tito_Scwrl_2QIO.pdb: