Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAESTTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEYAPKAVENFMTHAKDGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFE-------------TEINNHLYNIRGALSMARSQDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGH-YTVFGQVIEGMDVVDAIANIETDS-SDKPKEEVKIDKINILQEAKQK 3S6M Chain:A ((5-163)) ----------------------------------------------------------------------MVELHTNHGVIKLELDEAKAPKTVENFLNYVKKGHYDGTIFHRVINGFMIQGG---------------GFEPGLKQKPTDAPIANEANNGLKNDTYTIAMARTNDPHSATAQFFI--NVND---------------NEFLNHSSPTPQGWGYAVFGKVVEGQDIVDKIKAVKTGSHQDVPNDDVVIEK----------

General information: TITO was launched using: RESULT: Template: 3S6M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 710 -5087 -7.16 -36.08 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -7.16 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.496

(partial model without unconserved sides chains): PDB file : Tito_3S6M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3S6M-query.scw PDB file : Tito_Scwrl_3S6M.pdb: