Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNDFTTEIVQTLV--TKGDLNELFRSHLEKAINTLLRTELTAFLDYEKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYGHH-----YTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKRFSTGNRYISFNVY 5HJQ Chain:A ((78-367)) ALRSYTEIQQLLQQGKKRDVKNILRENSWP-INSPIRAQLWPMLCGQHLD-----------GFYWEMVHQVFGTTELS--------EKPIMLPAF------VDATHCLPYHLTSTGRAVADRIVNVLGYDCPDITYSP-VLYPITSILLHFMS---------------EEEAYICLAGLVGSKEKVFINQTKLQHEVTWKTVMQIAKKHTKSATSYFQRICPGLKLERIFM----------DWCWWILAGLPFQHL-VRIMDCYFHE------------------------------GI-------------KVLYRVALVILNLFHKECQSN--------------NEWSPDNIKNDIGNALIKFCKKIPVS-PAKLLHAAFSIRGLSTQYISR---

General information: TITO was launched using: RESULT: Template: 5HJQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1263 -17701 -14.02 -63.90 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -14.02 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.211

(partial model without unconserved sides chains): PDB file : Tito_5HJQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HJQ-query.scw PDB file : Tito_Scwrl_5HJQ.pdb: