Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------M-RLFIDGDGSP---VKEDVIKLGESYGLDVVIVTSVDHYTTKEYANHVQIV-YVDRGADSADYKIVGMVKKNDFL---------ITQ-DYGLASLVLPKGVRVFH-HSGK--------EFDEANIGTLLEQRYHSGK--------LRKA-GI-RTKGPKP-----FTEKDHMSFRQALKKAID-------- 5T76 Chain:A ((8-215)) AEAQKALRKAESLKKCLSVMEAKVKAQTAPNKDVQREIADLGEALAT--AVIPQWQKDELRETLKSLKKVMDDLDRASKADVQKRVLEKTKQFIDSNPNQPLVILEMESGATAKALNEALKLFKMHSPQTSAMLFTVDNEAGKITCLCQVPQNAANRGLKASEWVQQVSGLMDGKGGGKDVSAQATGKNVGCLQEALQLATSFAQLRLGD

General information: TITO was launched using: RESULT: Template: 5T76.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 435 29879 68.69 208.94 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 68.69 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.073

(partial model without unconserved sides chains): PDB file : Tito_5T76.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5T76-query.scw PDB file : Tito_Scwrl_5T76.pdb: