Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDK--DEAHCIHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL 3EFA Chain:A ((4-146)) -MKII-FSASPANRAAAYALRQAVFVEERGISADVEFDVKDTDQCEYAVLYLQPDL-PITTLRLEPQADHVMRFGRVCTRKAYRGHGWGRQLLTAAEEWATQRGFTHGEIHGELTAQRFYELCGYRVTAGPYDEDGAPVVIMHKQL

General information: TITO was launched using: RESULT: Template: 3EFA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 566 -17064 -30.15 -121.02 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -30.15 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.364

(partial model without unconserved sides chains): PDB file : Tito_3EFA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EFA-query.scw PDB file : Tito_Scwrl_3EFA.pdb: