Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MREYKGLKIAVAGTGYVGLSIATLLAQ-SHTVTAVDIIPEKVELINNKKSPIQDDYIEMYLAK--KELDLMATVDGVSAYKDADFVVIAAPTNYDSKKNFFDTSAVEAVIKLVMEV-N---PEAIMVIKSTIPVGYTASIREKT----------GSKNIIFSPEFLRESKALYDNLYPSRIIVGTDMEDERLVEAAQTFAKLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQQIINGVCRDPRIG--THYNNPSFGYGGYCLPKDTKQLLANYE--DVPENLIEAIVESNRTRKNFIADRVLELAGAYEANSHWDASKEKKVVIGVYRLTMKSNSDNFRQSSIQGVMKRVKAKGTTVVIYEPSLKDGEIFF--------------GSKVVNDLDQFKAMCDSIIANRYDTCLDDIK-------EKVYTRDIFQRD 1MUU Chain:A ((1-425)) ------MRISIFGLGYVGAVCAGCLSARGHEVIGVDVSSTKIDLINQGKSPIVEPGLEALLQQGRQTGRLSGTTDFKKAVLDSDVSFICVGTPSKKN-GDLDLGYIETVCREIGFAIREKSERHTVVVRSTVLPGTVNNVVIPLIEDCSGKKAGVDFGVGTNPEFLRESTAIKDYDFPPMTVIGELDKQ-----TGDLLEEIYRELD----APIIRKTVEVAEMIKYTCNVWHAAKVTFANEIGNIAKAVGVDGREVMDVICQDHKLNLSRYYMRPGFAFGGSCLPKDVRALTYRASQLDVEHPMLGSLMRSNSNQVQKAFDLITS---------------HDTRKVGLLGLSFKAGTDDLRESPLVELAEMLIGKGYELRIFDRNVEYA-RVHGANKEYIESKIPHVSSLLVSDLDEVVASSDVLVLGNGDELFVDLVNKTPSGKKLVDLVGFMPH-

General information: TITO was launched using: RESULT: Template: 1MUU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1778 -10153 -5.71 -26.51 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -5.71 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.480

(partial model without unconserved sides chains): PDB file : Tito_1MUU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MUU-query.scw PDB file : Tito_Scwrl_1MUU.pdb: