Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDLPNPVLAKVTERVIARSQKTRSAYLQRIEHAQGKFPARGALSCANLAHGFAGMEDNEKLIIKVGREPNIGIVSSYNEMLSAHAPYKTFPDIIKTAARENGGVAQFAGGVPAMCDGITQGNASM-ELSLFSRETIAMGTAIAL--SHNMFDAALCLGVCDKIVPGLLIGALQFGYLPTIFVPAGPMSSGLSNDDKAKIRQQFATGQVGRDALLEAESAAYHGQGTCTFYGTANSNQMLMEVMGLHLPSAAFVHPHTPLRDALTAEAAKRVLDLTAEQGNYTPIGHVIDEKAIINGIVALLATGGSTNHTLHLIAIAHAAGILIDWDDFDELSAVVPLLAKIYPNGKADVNH-FQAAGGVAFLIRNLLEAGLLHNDVKTVAGKGLQHYTKEPKLI-DGKLTWV---DGVVQSLDDKVLRSIDAPFQPDGGLRLMQGRLGRGVIKISAVAPEHRKVKAPAIVFDSQEAVQAAFDRGELHRDFIAVVRFQGARANGMPELHRLTPVLG-VLQDQGFHVALVTDGRMSGASGKVPAVIHLSPEALLNGPIGKVQTGDMLIIDAEAGVLDIELDEQTWQSRPVAQPEHQAENEVGFGRELFGVFRAAAAPAEHGASVFGALVGEEPQGQI---- 4R5O Chain:A ((2-427)) DDCEDLHLGNLA---------------HYPNVLKGTFPTES-----------QVLELGETLEITPELLNPEGATYSWLVNGKEYSTEPTFSYKIDNPC-----RADLSCIIKN------KYGKVEMSTSFSSNHNFSKGFFYVADGTFNFYDTEK-----------------KTAYQDCYASLNAGKTLGIGNYDSANIIHS--------------------NGKFYLLVGTSTSN-----------------RDHFYIVDAKTLY-----------YENSAVVGANLSGLTILNEQYGLVTGDG-----IRRIDLKSLNNVRIKNERL-----LCFYNSIIY-NGKVLSNDTYKDESKVKYYDVNELIA----------AKEGEAPAVTELDIIQKQKINFVLAKDGNVYTLESA--------------------DNGCNIVKIK--------------------------------NDFTLEKVFANFQPAKGP--YHSSPTIGMVASETENIIYLVSTD------GAIYKYI-LGDSDSLKAPFIAAESG--VSITAP-----LQLNQQSGELYVTYTEERKDESKIVVY-------------SKDGKVLHTVDCGESVPSQILFNN

General information: TITO was launched using: RESULT: Template: 4R5O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2193 46955 21.41 113.69 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 21.41 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.159

(partial model without unconserved sides chains): PDB file : Tito_4R5O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R5O-query.scw PDB file : Tito_Scwrl_4R5O.pdb: