Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIMQIRQFQAQRMQAPRDFTPIENTPICVEIGAGKGKHALLFSGQNPQHTLYAIERTREKFLAMQKQHGLE----PRDNLIPVHADALPWVVHALY-PAQVEHFFILYPNPEPHNPAQRWLNMPFFEFLLSRLKTGGTITLAGNIPEYIEEAEQQLLNVWKLPYQKEKIAQTSARTHFEIKYLERGELCQQLIMTKPEGYNTRFDEFAPLQGQIHVE 5BW4 Chain:B ((46-164)) ----------------------SAERILVDVGTGDARTAYRQAIAHPEWLVVGVDPAWQRMTETAVRAARKPAKGGAPNLVLVSSAIETVP---AALHGVADEVMVLMPWGKLLRGV-VLGEADVLSGLRAVAKPGAPLEISIGT------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5BW4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 544 -33017 -60.69 -289.62 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : -60.69 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.420

(partial model without unconserved sides chains): PDB file : Tito_5BW4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5BW4-query.scw PDB file : Tito_Scwrl_5BW4.pdb: