Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAQALVLTDNAANKVRQLRDSEGNDDLMLRVYVTGGGCSGFSYGFNFAESINEDDAEFVNGDVKMLVDSLSYQYLVGSVVDYVEGLEGSRFIVQNPNATTTCGCGSSFSI 4X33 Chain:A ((23-33)) ----------------------------------------------------------------------------------------------------YPCPCGDRFQI

General information: TITO was launched using: RESULT: Template: 4X33.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 10 -2097 -209.70 -190.64 target 2D structure prediction score : 0.82 Monomeric hydrophicity matching model chain A : 0.52 3D Compatibility (PKB) : -209.70 2D Compatibility (Sec. Struct. Predict.) : 0.82 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.874

(partial model without unconserved sides chains): PDB file : Tito_4X33.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4X33-query.scw PDB file : Tito_Scwrl_4X33.pdb: