Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIRDVNKIILHKKDSILKALELLDLYALRIVLVVDDHNHLIGSITDGDIRRGLLKGQDVHASVETIMHTTPYSIEEGSLNNRQIFEIMREKSYLALPVIKNNQLVNIITLDDLIRKKRKENPVFIMAGGFGTRLRPLTDTCPKPMLPVGGKPLLETIISSFKNQGFYKFYISTHYLPEVINEYFGDGEKLGVQIQYVHETDPLGTGGALSLLPASDI-KLPFIVINGDVLTNMNFEKLLEFHEKRDAIATMCVREFQYQIPYGVVNSE--DHVIQSMTEKPSYFFDINTGIYVISPELLPEVNAQFIGMPTILEQQMEKQNKVLSYPLHEYWLDIGHMEDYNRAQRDIINLDFGKF 4Y7U Chain:A ((1-223)) ------------------------------------------------------------------------------------------------------------------------MKAMILAAGKGERMRPLTLHTPKPLVPVAGQPLIEYHLRALAAAGVTEVVINHAWLGQQIEDHLGDGSRFGLSIRYSPEGEPLETGGGIFKALP-LLGDAPFLLVNGDVWTDYDFARLQAPLQG--L-AHLVLVD--NPGHHGRGDFRLVGEQVVDGDDAP--GTLTFSGISVLHPALFEGCQAGAFKLAPLLRQAMAAG-KVSGEHYRGHWVDVGTLERLAEAESLIGERA----

General information: TITO was launched using: RESULT: Template: 4Y7U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1189 -70704 -59.47 -321.38 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -59.47 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.417

(partial model without unconserved sides chains): PDB file : Tito_4Y7U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Y7U-query.scw PDB file : Tito_Scwrl_4Y7U.pdb: