Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIGQYRVTALIPARGGSKRLPRKNVKLLVDKPLIAWSIEVAKASKYIDRVVVSTDDEEIKQVSEQYGAEVPFLRPEYLSNDHASSFDVIKHAIDFLHLGQKNELIVLLQPTSPLRLVSELDTALEFFIAKN-AKGIVSISETEHSP------MWSNT-LPENGCMSDFIRPEVQGKRS------QDL--PKFFRLNGSIYIYETLCLLEQ-----S-----K-I--F---F-NENVYGFETSLKTAIDIDTDLDFLIAETIMKNRAIE 4XWI Chain:A ((11-261)) ----QAFTVVIPARYASTRLPGKPLQDIAGQPMIQRVWNQARKSA-ASRVVVATDDERILAACQGFGAEALLTRAEHNS-GTD----RLEEVASRLGL-ASDAIVVNVQGDEPLIPPALIDQVAANLAAHPEAAIATLAEPIH-EVSALFNPNVVKVATDIDGLALTFSRAPLPWARDAFARDRDSLPEGVPYRRHIGIYAYRVGFLADFVAWGPCWLENAESLEQLRALWHGVRIHVADARENMLPGVDTPEDLERVRRVLG-----

General information: TITO was launched using: RESULT: Template: 4XWI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1054 -59945 -56.87 -274.97 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -56.87 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.466

(partial model without unconserved sides chains): PDB file : Tito_4XWI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XWI-query.scw PDB file : Tito_Scwrl_4XWI.pdb: