Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAPVALTPELLTQLTAIVGENRIKTD-ADS------LENWGKDHTKHF---NPNPSVIVFPSTTEQVQEVVKLANQFNIAITPSGGRTGLSAGA-VA-TKGEIVISMD-KMNQILEFFPADRMVRVQAGVVTEQLQNYAEEQGMYYPVD---FASAGSSQIGGNIGTNAGGIKVIKYGMTRNWVLGLTVVTGKGDILRLNKGMI-------------------------KNATGYALQHLFIGGEGTLGLVTEAEIKLERQPQNLQVLVLGVPDFDAVMPVLHAFQ---KDIDLTAFEFFGELTMQKVLDRG-----HVQRP-----------FETQCPFYVLLEFEAPYEPIIDKAMEIFEHCMEQGWVLDGVMSQSLDQVESLWRLREDISESIAP----FIPYKND----ISVLITHVPA-----FIREIDAIVQENYPYFEICWFGHIGDGNLHLNILKPENLTKDEFFAKCQVVNKYVFDTVKKYDGSISAEHGVGMTKKPYLEYSRSAE---EIEYMKALKKVFDPKGIMNPGKLFDL 1E8G Chain:B ((17-548)) ---SLSDFNEFIQDIIRIVGSENVEVIS---VDGSYMKPTHTHDPTHVMDQDYFLASAIVAPRNVADVQSIVGLANKFSFPLWPISIGRNSGYGGAAPRVSGSVVLDMGKNMNRVLEVNVEGAYCVVEPGVTYHDLHNYLEANNLRDKLWLDVPDLGGGSVLGNAVE---RGVGYTPYGDHWMMHSGMEVVLANGELLRTGMGALPDPKRPETMGLKPEDQPWSKIAHLFPYGFGPYIDGLF--SQSNMGIVTKIGIWLMPNPGGYQSYLITLPKDGDLKQAVDIIRPLRLGMALQNVPTIRHILLDAAVLGDKRSYSSRTEPLSDEELDKIAKQLNLGRWNFYGALYGPEPIRR-VLWETIKDAFSAIPGVKFYFPEDTPENSVLRVRDKTMQGIPTYDELKWIDWLPNGAHLFFSPIAKVSGEDAMMQYAVTKKRCQEA--GLDFIGTFTVGMREMHHIVCIVFNKKDLIQKRKVQWLMRTLIDDCAANGW---GEYRTHLAFMDQIMETYNWNNSSFLRFNEVLKNAVDPNGIIAPGKSGV-

General information: TITO was launched using: RESULT: Template: 1E8G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2507 -26039 -10.39 -57.74 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : -10.39 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.394

(partial model without unconserved sides chains): PDB file : Tito_1E8G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E8G-query.scw PDB file : Tito_Scwrl_1E8G.pdb: