Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTRHFSQLPKAALAYPKVVQGLIFKKPKGPKILPQVEYVVDTLEIDQNHLKAYNEVCGFQNN-----GFV-PAIYLAVLSQSLQMHMMTA----EA--FPFPILGLVHIRNQIKQTRPI-GVTEKLTLSCKLGELKPHDKGVQFDFITTAKV--GNEVVMEGLTTYLSRQKVEKRVGEKAKE--------EQA--PAYVPKAEWNILENTGRRYAKVSGDFNLIHIHAITAKAFGFKQAIAHGMWSKAKALANLE--L-----P-NAYEADVWFKLPMFLPSKVEFLTANAD---KKTDFLIRNAKSQKPHVAGTVKAL 4V8V Chain:B ((1067-1578)) ----------------------------------------TVTVDWDPERVADHTGVTAT-FGAPLAPTLTVVPDALVGRCWPAVFAAIGSAATEAGFPVIEGLLSLVHLDHAARLLAELPKEPAEFTVTAKASAATDTEVGRVVPVSVEVRNAADGALLATLEERFAIRGRTGAAELTDPVRAGGAISDNATDTPRRRRRDVTVG-APVDMRPFAVVSGDHNPIHTDRAAALLAGLEGPIVHGMWLSAAAQHVVTATDGKPVPPAKLIGWTARFLGMVKPGDQVDFRVDRVGIDVGAEVLEVSARIGSELVMAATA---

General information: TITO was launched using: RESULT: Template: 4V8V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1119 7347 6.57 30.74 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain B : 0.60 3D Compatibility (PKB) : 6.57 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.323

(partial model without unconserved sides chains): PDB file : Tito_4V8V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4V8V-query.scw PDB file : Tito_Scwrl_4V8V.pdb: