Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNQLRIIGGEWKRRVLPFASIEGLRPTPDRVRETLFNWLMWNIQNAHVLDICTGSGALGFEALSRGAASVYMIE-PDKTQARFLKDNIQLLKAHNCHLINATA-QQALPRLKEQFDVVFLDPPYSLNLWQELAHLADSH--IKNNGYIYVEADRNLSELHLPATWQQIKSTKAGTVHAGLYQKVSE 2FPO Chain:D ((12-194)) --GQIRIIGGQWRGRKLPVPD----SPGTDRVRETLFNWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATL-KAGNARVVNSNAMSFLAQK-GTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEVENGLPTVPANWSLHREKVAGQVAYRLYQREAQ

General information: TITO was launched using: RESULT: Template: 2FPO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 939 -4219 -4.49 -24.11 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain D : 0.76 3D Compatibility (PKB) : -4.49 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.659

(partial model without unconserved sides chains): PDB file : Tito_2FPO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FPO-query.scw PDB file : Tito_Scwrl_2FPO.pdb: