Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRIKFNMVAKKLALILCSLSFLQTSFAGFERFWIFSKDANTQVDDTWNAL-SEEEQRAVIKRYQSLKELPSNQSANLQQRMDWFNQLPDAEKQKMREAWQKMSTQERNDLRQRMLKASADERTAIREEYINKYLEH 2GTS Chain:A ((4-80)) ------------------------------------------------VQMDTEEVREFVGHLERFKELLREEVNSLSNHFHNLESWRDARRDKFSEVLDNLK-STFNEFDEAAQEQIAWLKERIR----------

General information: TITO was launched using: RESULT: Template: 2GTS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 108 22273 206.23 293.06 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 206.23 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.454

(partial model without unconserved sides chains): PDB file : Tito_2GTS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GTS-query.scw PDB file : Tito_Scwrl_2GTS.pdb: