Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYQINALNPKDEGHKARKRFGQNFLHDQRVIAKIVRSVNPRTGDNIVEIGPGLAALTSPLIGECDALTVVELDRDLAAGLPERVPHPERLTIVEADALKYDFSQLVKDGRPLRVVGNLPYNISTPLLFHLLEFGSQVKDMHFMLQKEVVERITAEPNTKEYGRLSVMIQYYCQPTFLFEVPAGAFNPPPKVTSAVFRLVPYEQKPITAKDEKALARLVAHVFTQRRKTLRNSLKG--MLA---EDGFEKAGVDPMARPETLTLAEFVALADQMVA 3FUX Chain:C ((15-268)) ---------ERHGLFADKRFGQNFLVSEAHLRRIVEAARP-FTGPVFEVGPGLGALTRALLEAGAEVTAIEKDLRLRPVLEETLS-GLPVRLVFQDALLYPWEE---VPQGSLLVANLPYHIATPLVTRLLKT-GRFARLVFLVQKEVAERMTARPKTPAYGVLTLRVAHHAVAERLFDLPPGAFFPPPKVWSSLVRLTPTGAL-----DDPGLFRLVEAAFGKRRKTLLNALAAAGYPKARVEEALRALGLPPRVRAEELDLEAFRRLREGLE-

General information: TITO was launched using: RESULT: Template: 3FUX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1256 -139562 -111.12 -560.49 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain C : 0.77 3D Compatibility (PKB) : -111.12 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.507

(partial model without unconserved sides chains): PDB file : Tito_3FUX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FUX-query.scw PDB file : Tito_Scwrl_3FUX.pdb: