Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLSASLQAIDIDDSIQLEQDGSILYLWLNRPESRNAMNLNMVNAIQQVFTAIRDDLSIRAVIIRGEGGTFCAGGDIKDMAALRVEATNVGSLQPYTDFNRRFGAMLEQVEAAPQTVVVILEGAVLGGGFGLACVSDVAISRDNAQFGLPETGLGVIPAQIAPFVVKRIGLTQARRLALLGMRFEGHTALSVGVVHQIAHNEIELEQALHETIQHIKRAAPQASRVTKALLHRTLNEPLNELLDDAAQQFAQAVGGAEGQEGTMAFIQKRLPNWADES 4NNQ Chain:B ((12-257)) -------------RGVRLTAEPHVLRATLTSPDGLNSLSGAALDALGAALDRAEADPECRVLLLEGSGGTFCTGLD---------------------RGGAEFLALMRRFGETPLAVVACVDGRAAGGGVGLAAAADLVIATERSEFSLPEALWGLVPCCVLPVLVRRTGFQPAYAMALSTQPVSARRAADFRLVDEVVPDP-D--AAVRRLLVRLTRLDPATIGELKQYFRAMWF-TTEDTDAFALREFTRLIDSPVARRRITDYTT----------

General information: TITO was launched using: RESULT: Template: 4NNQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1229 -160883 -130.91 -699.49 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.73 3D Compatibility (PKB) : -130.91 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.503

(partial model without unconserved sides chains): PDB file : Tito_4NNQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NNQ-query.scw PDB file : Tito_Scwrl_4NNQ.pdb: