Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence PHFCEVSVSYENANKQKITTATKYVVPPGSVDIAVGSQMYAVTVDETCKRTDSISIPGR-FKIDTQGKKLEGN---PTENYIRSVVLQLNHRGLRMVSRPAP 1NPS Chain:A ((1-88)) ---ANITVFY-NEDF----QGKQVDLPPGNYT---RAQLAALGIEN--NTISSVKVPPGVKAILYQNDGFAGDQIEVVANAEELGPLNNNVSSIRVISVPV-

General information: TITO was launched using: RESULT: Template: 1NPS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 397 -10943 -27.56 -130.27 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -27.56 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.268

(partial model without unconserved sides chains): PDB file : Tito_1NPS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NPS-query.scw PDB file : Tito_Scwrl_1NPS.pdb: