Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ECWVELFGPGKVSHGKQTGRWNAPYYFNLD-P-MRLIYLHENCKVNEKHTVLLPDNV-KW-DVETGMIS------ 1CSE Chain:I ((8-70)) KSFPEVVG---KT-VDQ-----AREYFTLHYPQYNVYFLPEGSPVTLD---LRYNRVRVFYNPGTNVVNHVPHVG

General information: TITO was launched using: RESULT: Template: 1CSE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain I - contact count / total energy / energy per contact / energy per residue : 128 -9868 -77.09 -186.18 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain I : 0.72 3D Compatibility (PKB) : -77.09 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.206

(partial model without unconserved sides chains): PDB file : Tito_1CSE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1CSE-query.scw PDB file : Tito_Scwrl_1CSE.pdb: