Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence CLGVLLRNDGR----KPILGFEPFRIRRTVSFPGYDFVLKVGRRCITEKISGTIPPHL-HVHVVWGFPAPVR-PVCNV--YNGWTERFERYGRNVREAPW 4NOO Chain:B ((28-122)) CNDTSGVHQKILVCIQNEIAKSETQIRNNISSKSIDYGFPDD---FYSKQRLAIHEKCMLYINVGGQRGELLMNQCELSMLQGLDIYIQQYIEDVDNS--

General information: TITO was launched using: RESULT: Template: 4NOO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 203 -4248 -20.93 -48.83 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain B : 0.62 3D Compatibility (PKB) : -20.93 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.177

(partial model without unconserved sides chains): PDB file : Tito_4NOO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NOO-query.scw PDB file : Tito_Scwrl_4NOO.pdb: