Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLHGKRKEIYKYEAPWTVYAMNWSVRPDKRFRLALGSFVEEYNNKVQLVGLDEESSEFICRNTFDHPYPTTKLMWIPDTKGVYPDLLAT-SGDYLRVWRVGETETRLECLLNNNKNSDFCAPLTSFDWNEVDPYLLGTSSIDTTCTIWGLETGQVLGRVNLVSGHVKTQLIAHDKEVYDIAFSRAGGGRDMFASVGADGSVRMFDLRHLEHSTIIYEDPQHHPLLRLCWNKQDPNYLATMAMDGMEVVILDVRVPCTPVA-------RLNNHRACVNGIAWAPHSSCHICTAADDHQALIWDIQQMPRAIEDPILAY-TAEGEINNVQWASTQPDWIAICYNNCLEILRV 5MQF Chain:F ((64-356)) -------------GHEGEVYCCKFH--PNGS-TLASAGF----DRLILLWNVYGDCDNY--ATLKGHSGAVMELHYNTDGSM----LFSASTDKTVAVWDSETGERVKR--LKG-----HTSFVNSCYPARRGPQLVCTGSDDGTVKLWDIRKKAAIQT------------FQNTYQVLAVTFNDTS---DQIISGGIDNDIKVWDLRQNKLTYTMR--GHADSVTGLSLSS-EGSYLLSNAMDN-TVRVWDVRPFAPKERCVKIFQGNVHNFEKNLLRCSWSPDGS-KIAAGSADRFVYVWDTTSRRI-----LYKLPGHAGSINEVAFHPDEPIIISASSDKRLYMGEI

General information: TITO was launched using: RESULT: Template: 5MQF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 1817 -54590 -30.04 -192.22 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain F : 0.70 3D Compatibility (PKB) : -30.04 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.371

(partial model without unconserved sides chains): PDB file : Tito_5MQF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MQF-query.scw PDB file : Tito_Scwrl_5MQF.pdb: