Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIHFSINKNLFLQALNITKRAISSKNAIPILSTVKIDVTNEGVTLIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQNQIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRPILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKN--SDDFDVVIPSRSLREFSAVFTDDIETVEIFFAN-------NQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMERARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLIDSLKALNSEKVTISFISAVRPFTLVPADT--------------DEDFMQLITPVRTN 5AGV Chain:A ((16-405)) DLTFRLLRESFADAVSWVAKNLPARPAVPVLSGVLLTGSDNGLTISGFDYEVSAEAQVGAE-------IVSPGSVLVSGRLLSDITRALPNKPVDVHVEGN-RVALTCGNARFSLPTMPVEDYPTLPTLP-EETGLLPAELFAEAISQVAIAAGRDDTLPMLTGIRVEIL-GETVVLAATDRFRLAVRELKWSASSPDIEAAVLVPAKTLAEAAKAGIGG-SDVRLSLGTGPGVGKDGLLGISGNGKRSTTRLLDAEFPKFRQLLPTEHTAVATMDVAELIEAIKLVALVADR-GAQ-VRMEFADGSVRLSAGADDVGRAEEDLVV-DYAGEPLTIAFNPTYLTDGLSSLRSERVSFGFTTAGKPALLRPVSGDDRPLNGNGPFPAVSTDYVYLLMPVRLP

General information: TITO was launched using: RESULT: Template: 5AGV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1944 -97199 -50.00 -267.03 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -50.00 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.518

(partial model without unconserved sides chains): PDB file : Tito_5AGV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5AGV-query.scw PDB file : Tito_Scwrl_5AGV.pdb: