Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAK----REEIAAFLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPATYLKKDVATVLVSFARSGNSPESVATVDLAKSLVDEL-YQVTITCATDGKLALQAHGDDRNLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFA---------VKSERFEVVSSLARKVLDKAEDVKELVD--LDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSLINDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGL-ENVKEVALGCGGVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK 1JXA Chain:C ((241-607)) -----DAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQVDLSELGPNADELLS--KVEHIQILACGTSYNSGMVSRYWFESLAG---IPCDVEIASEFRYRK-SAV--RRNSLMITLSQSGETADTLAGLRLSKEL--GYLGSLAICNVPGSSLVRESD-----LALMTNAG-TEIGVASTKAFTTQLTVLLMLVAKLSKLKGLDASIEHDIVHGLQALPSRIEQMLSQDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISY--IHAEAYAAGELKHGPLALIDADMPVIVVAPNNE-LLEKLKSNIEEVRARG--GQLYVFADQDAGFVSSDNMHIIEMP-HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTV-----------

General information: TITO was launched using: RESULT: Template: 1JXA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 2134 -10454 -4.90 -29.87 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain C : 0.71 3D Compatibility (PKB) : -4.90 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.430

(partial model without unconserved sides chains): PDB file : Tito_1JXA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JXA-query.scw PDB file : Tito_Scwrl_1JXA.pdb: