Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTYEYKSHIYLAETALNVKDLASQTAFYQQVIGLEILSQTETESILGLGGK--VLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA---DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEPFILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVP------SASWIAAGDYHHHLAVNEWRGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDA--PIKWM-------TSSKLEITDSDGIVTRIRLAR 1F1U Chain:A ((9-271)) -SVPAPDIVRCAYMEIVVTDLAKSREFYVDVLGLHVTEEDENTIYLRSLEEFIHHNLVLRQ------GPIAAVAAFAYRVKSPAEVDAAEAYYKELGCRTERRKEGFTKGIGDSVRVEDPLGFPYEFFYETEHVERLT-----Q-----R-----Y-------DLYSAGELVRLDHFNQVTPDVPRGRAYLED-LGFRVSEDIKDSDGVTYAAWMHRKQTVHDTALTG----------GNGPRMHHVAFATHEKHNIIQICDKMGALRISDRIERGPGRHGVSNAFYLYILDPDGHRIEIYTQ-

General information: TITO was launched using: RESULT: Template: 1F1U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1244 -8033 -6.46 -33.06 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -6.46 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.407

(partial model without unconserved sides chains): PDB file : Tito_1F1U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1F1U-query.scw PDB file : Tito_Scwrl_1F1U.pdb: