Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVVVDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKVLGCEGVIVTNAAGGIG--FGPGTLMAISDHINM---TGQNPLMGENLDDFGPRFPDMSRAYTPEYRATAHEVAKKL--NIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVAAHSGLKVLGISCITNFAAGFQEEL---NHEEVVEVTERVKGDFKGLLKAILAEL 1PWY Chain:E ((12-281)) ---------TAEWLLSHTKHRPQVAIICGSGLGGLTDKLTQAQIFDYSEIPNFPRSTVPGHAGRLVFGFLNGRACVMMQGRFHMYEGYPLWKVTFPVRVFHLLGVDTLVVTNAAGGLNPKFEVGDIMLIRDHINLPGFSGQNPLRGPNDERFGDRFPAMSDAYDRTMRQRALSTWKQMGEQRELQEGTYVMVAGPSFETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESLEKANHEEVLAAGKQAAQKLEQFVSILMASI

General information: TITO was launched using: RESULT: Template: 1PWY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1492 -87792 -58.84 -337.66 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain E : 0.83 3D Compatibility (PKB) : -58.84 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.517

(partial model without unconserved sides chains): PDB file : Tito_1PWY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1PWY-query.scw PDB file : Tito_Scwrl_1PWY.pdb: