Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGLAANMIGVQKRVIIFNLGLVP-VVMFNPVLLSF-EGSYEAEEGCLSLVGVR-STKRYETIRLAYRDSKWQEQTITLTGFPAQICQHELDHLEGRII 5VCP Chain:D ((39-158)) -------------------------LVKDMFETM--HDANGAGLAAPQIGVNLQVVIFGF-PVPETVLINPTITPVSQDMEEGWEGCLSVPGLRGAVSRFSMIKYHGFDQYGKPIDRVAEGFHARVVQHECDHLIGKL-

General information: TITO was launched using: RESULT: Template: 5VCP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 489 -55121 -112.72 -515.15 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain D : 0.71 3D Compatibility (PKB) : -112.72 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.455

(partial model without unconserved sides chains): PDB file : Tito_5VCP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5VCP-query.scw PDB file : Tito_Scwrl_5VCP.pdb: