Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVTFFIGEERLKIKIGLASICLLGLATSHVAANETEVAKTSQDTTTASSSSEQNQSSNKTQTSAEVQTNAAAYWDGDYYVKDDGSKAQSEWIFDNYYKAWFYINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGNKWYYFKKWGYMAKSQWQGSYFLNGQGAMIQNEWLYDPAYSAYFYLKSDGTYANQEWQKVGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLNVGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENKVDGVIVRLGYS-GKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNM-NLSYPIYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQA-GYQNVYVYSYRSLLQTRLKHPDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY 4KRT Chain:A ((16-208)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NNNNLKGIDVSNWKGNI-NFESVKN-DGVEVVYIKATEGNYFKDKYAKQNYEGAKEQGLSVGFYHFFRANK--GAKDQANFFIDYLNEIGAVNYDCKLALDIETTEG--------VGVRDLTSMCIEFLEEVKRLTGKE-VVVYTYTSFANNNLD-SRLGNYPVWIAHYGVNTPGANNIWSSWVGFQYSENGSVAGVNGGCDMNEFT

General information: TITO was launched using: RESULT: Template: 4KRT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1028 171 0.17 0.90 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 0.17 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.574

(partial model without unconserved sides chains): PDB file : Tito_4KRT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4KRT-query.scw PDB file : Tito_Scwrl_4KRT.pdb: