Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVKDRIALSMIEKAEQDGILKLG-STIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVERRKIIQAYGAELVLTPGSEGMKGAIAKAQEI-AAERDGFLPLQFDNPANPEVHERTTGAEILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPHKIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIEVAKKLGT-GKKVLALAPDNGERYLSTALYEL 4AIR Chain:B ((31-335)) -NVAQSIDQLIGQTPALYLNKL-NNTKAKVVLKMECENPMASVKDRLGFAIYDKAEKEGKLIPGKSVVVESSSGNTGVSLAHLGAIRGYKVIITMPESMSLERRCLLRIFGAEVILTPAALGMKGAVTMAKKIVTANPNAVLADQFATKYNALIHEETTGPEIWEQTNHN-VDCFIAGVGTGGTLTGVARALKKMGSHARIVAVEPME--------------------------DRSLIDEVFCVAGDDAIETALKLTRSDGVFCGFSGGANVYAALKIAERPEMEGKTIVTIIPSFGERYLSTALYR-

General information: TITO was launched using: RESULT: Template: 4AIR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1564 -5602 -3.58 -20.30 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -3.58 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.493

(partial model without unconserved sides chains): PDB file : Tito_4AIR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4AIR-query.scw PDB file : Tito_Scwrl_4AIR.pdb: